Protein

## Lalb_04502

Length
2,385 amino acids

### Protein family membership

- Pre-mRNA-processing-splicing factor 8 (IPR027652)

### Homologous superfamilies

1. Homologous superfamily
   1,036 - 1,292
   - Ribonuclease H-like superfamily (IPR012337)
   1,813 - 2,065
   - Ribonuclease H-like superfamily (IPR012337)
2. Homologous superfamily
   1,223 - 1,342
   - Pre-mRNA-processing-splicing factor 8, U5-snRNA-binding domain superfamily (IPR042516)
   1,811 - 1,971
   - Prp8 RNase domain IV, palm region (IPR043172)
   1,972 - 2,070
   - Prp8 RNase domain IV, fingers region (IPR043173)

1 500 1000 1500 2000 2385

### Domains and repeats

1. Domain
   98 - 249
   - PRO8NT domain (IPR012591)
   1,025 - 1,115
   - RNA recognition motif, spliceosomal PrP8 (IPR019582)
   1,810 - 2,066
   - PRP8 domain IV core (IPR021983)
   2,262 - 2,382
   - PROCT domain (IPR012984)
2. Domain
   435 - 840
   - PROCN domain (IPR012592)
   1,249 - 1,382
   - Pre-mRNA-processing-splicing factor 8, U5-snRNA-binding (IPR019581)
   1,481 - 1,639
   - Pre-mRNA-processing-splicing factor 8, U6-snRNA-binding (IPR019580)
   2,149 - 2,284
   - MPN domain (IPR037518)
     - JAB1/MPN/MOV34 metalloenzyme domain (IPR000555)

1 500 1000 1500 2000 2385

### Detailed signature matches

1. IPR012337
   Ribonuclease H-like superfamily
   1. SSF53098 (Ribonucle...)
      1,036 - 1,292
      SUPERFAMILY
      SSF53098 (Ribonuclease H-like)
      1,813 - 2,065
      SUPERFAMILY
      SSF53098 (Ribonuclease H-like)
2. IPR042516
   Pre-mRNA-processing-splicing factor 8, U5-snRNA-binding domain superfamily
   1. G3DSA:1.20.58...
      1,223 - 1,342
      GENE3D
      G3DSA:1.20.58.1750 (G3DSA:1.20.58.1750)
3. IPR043172
   Prp8 RNase domain IV, palm region
   1. G3DSA:3.30.42...
      1,811 - 1,971
      GENE3D
      G3DSA:3.30.420.230 (G3DSA:3.30.420.230)
4. IPR043173
   Prp8 RNase domain IV, fingers region
   1. G3DSA:1.20.80.40
      1,972 - 2,070
      GENE3D
      G3DSA:1.20.80.40 (G3DSA:1.20.80.40)
5. IPR027652
   Pre-mRNA-processing-splicing factor 8
   1. PTHR11140 (PRE-MRNA...)
      51 - 2,384
      PANTHER
      PTHR11140 (PRE-MRNA SPLICING FACTOR PRP8)
6. IPR012591
   PRO8NT domain
   1. PF08082 (PRO8NT)
      98 - 249
      Pfam
      PF08082 (PRO8NT)
7. IPR012592
   PROCN domain
   1. PF08083 (PROCN)
      435 - 840
      Pfam
      PF08083 (PROCN)
8. IPR019582
   RNA recognition motif, spliceosomal PrP8
   1. PF10598 (RRM_4)
      1,025 - 1,115
      Pfam
      PF10598 (RRM_4)
9. IPR019581
   Pre-mRNA-processing-splicing factor 8, U5-snRNA-binding
   1. PF10597 (U5_2-snRNA...)
      1,249 - 1,382
      Pfam
      PF10597 (U5_2-snRNA_bdg)
10. IPR019580
    Pre-mRNA-processing-splicing factor 8, U6-snRNA-binding
    1. PF10596 (U6-snRNA_bdg)
       1,481 - 1,639
       Pfam
       PF10596 (U6-snRNA_bdg)
11. IPR021983
    PRP8 domain IV core
    1. cd13838 (RNase_H_li...)
       1,816 - 2,066
       CDD
       cd13838 (RNase_H_like_Prp8_IV)
    2. PF12134 (PRP8_domainIV)
       1,810 - 2,039
       Pfam
       PF12134 (PRP8_domainIV)
12. IPR037518
    MPN domain
    1. PS50249 (MPN)
       2,153 - 2,284
       PROSITE profiles
       PS50249 (MPN)
13. IPR000555
    JAB1/MPN/MOV34 metalloenzyme domain
    1. SM00232 (pad1_6)
       2,149 - 2,283
       SMART
       SM00232 (pad1_6)
    2. PF01398 (JAB)
       2,151 - 2,253
       Pfam
       PF01398 (JAB)
14. IPR012984
    PROCT domain
    1. PF08084 (PROCT)
       2,262 - 2,382
       Pfam
       PF08084 (PROCT)

no IPR
Unintegrated signatures

1. G3DSA:3.40.14... (C...)
   2,108 - 2,385
   GENE3D
   G3DSA:3.40.140.10 (Cytidine Deaminase)
2. G3DSA:3.90.15...
   1,620 - 1,802
   GENE3D
   G3DSA:3.90.1570.40 (G3DSA:3.90.1570.40)
3. PTHR11140:SF1
   51 - 2,384
   PANTHER
   PTHR11140:SF1 (PTHR11140:SF1)
4. cd08056 (MPN_PRP8)
   2,117 - 2,367
   CDD
   cd08056 (MPN_PRP8)

### Other features

1. Coil
   1,430 - 1,450
   COILS
   Coil (Coil)

### Residue annotation

1. active site cd13838
   1,831D
   active site
   1,831D, 1,832D, 1,914T
   CDD
   cd13838 (RNase_H_like_Prp8_IV)
   1,832D
   active site
   1,831D, 1,832D, 1,914T
   CDD
   cd13838 (RNase_H_like_Prp8_IV)
   1,914T
   active site
   1,831D, 1,832D, 1,914T
   CDD
   cd13838 (RNase_H_like_Prp8_IV)

### GO term prediction

#### Biological Process

GO:0000398 mRNA splicing, via spliceosome

#### Molecular Function

GO:0003723 RNA binding
GO:0005515 protein binding
GO:0008237 metallopeptidase activity
GO:0017070 U6 snRNA binding
GO:0030623 U5 snRNA binding
GO:0070122 isopeptidase activity

#### Cellular Component

GO:0005681 spliceosomal complex

Generated with InterProScan 5.45-80.0