Protein

## Lalb_10122

Length
558 amino acids

### Protein family membership

- Methylthiotransferase (IPR005839)
  - tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase MiaB (IPR006463)

### Homologous superfamilies

1. Homologous superfamily
   71 - 203
   - Methylthiotransferase, N-terminal domain superfamily (IPR038135)
   212 - 473
   - Radical SAM, alpha/beta horseshoe (IPR023404)

1 100 200 300 400 500 558

### Domains and repeats

1. Domain
   71 - 188
   - Methylthiotransferase, N-terminal (IPR013848)
   215 - 459
   - Radical SAM (IPR007197)
     - Elp3/MiaB/NifB (IPR006638)
   470 - 545
   - TRAM domain (IPR002792)

1 100 200 300 400 500 558

### Detailed signature matches

1. IPR038135
   Methylthiotransferase, N-terminal domain superfamily
   1. G3DSA:3.40.50...
      71 - 203
      GENE3D
      G3DSA:3.40.50.12160 (G3DSA:3.40.50.12160)
2. IPR023404
   Radical SAM, alpha/beta horseshoe
   1. G3DSA:3.80.30.20 (t...)
      212 - 473
      GENE3D
      G3DSA:3.80.30.20 (tm_1862 like domain)
3. IPR005839
   Methylthiotransferase
   1. SFLDG01061 (methylt...)
      68 - 549
      SFLD
      SFLDG01061 (methylthiotransferase)
   2. TIGR00089
      72 - 506
      TIGRFAMs
      TIGR00089 (TIGR00089)
4. IPR006463
   tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase MiaB
   1. SFLDF00273 ((dimeth...)
      69 - 545
      SFLD
      SFLDF00273 ((dimethylallyl)adenosine_tRNA)
5. IPR013848
   Methylthiotransferase, N-terminal
   1. PS51449 (MTTASE_N)
      71 - 188
      PROSITE profiles
      PS51449 (MTTASE_N)
   2. PF00919 (UPF0004)
      72 - 172
      Pfam
      PF00919 (UPF0004)
6. IPR007197
   Radical SAM
   1. PF04055 (Radical_SAM)
      219 - 415
      Pfam
      PF04055 (Radical_SAM)
7. IPR006638
   Elp3/MiaB/NifB
   1. SM00729 (MiaB)
      215 - 459
      SMART
      SM00729 (MiaB)
8. IPR002792
   TRAM domain
   1. PF01938 (TRAM)
      470 - 544
      Pfam
      PF01938 (TRAM)
   2. PS50926 (TRAM)
      470 - 545
      PROSITE profiles
      PS50926 (TRAM)
9. IPR020612
   Methylthiotransferase, conserved site
   1. PS01278 (MTTASE_RAD...)
      219 - 239
      PROSITE patterns
      PS01278 (MTTASE_RADICAL)

no IPR
Unintegrated signatures

1. PTHR43020 (FAMILY N...)
   34 - 555
   PANTHER
   PTHR43020 (FAMILY NOT NAMED)
2. PTHR43020:SF2 (CDK5...)
   34 - 555
   PANTHER
   PTHR43020:SF2 (CDK5 REGULATORY SUBUNIT-ASSOCIATED PROTEIN 1)
3. SFLDF00413 (CDK5RAP1)
   68 - 549
   SFLD
   SFLDF00413 (CDK5RAP1)
4. SFLDG01082 (B12-bin...)
   174 - 440
   SFLD
   SFLDG01082 (B12-binding_domain_containing)
5. SSF102114 (Radical ...)
   305 - 460
   SUPERFAMILY
   SSF102114 (Radical SAM enzymes)
   198 - 274
   SUPERFAMILY
   SSF102114 (Radical SAM enzymes)
6. TIGR01574
   72 - 500
   TIGRFAMs
   TIGR01574 (TIGR01574)

### Residue annotation

1. SFLDF00273
   80C
   Binds [4Fe-4S] cluster
   80C, 116C, 151C
   SFLD
   SFLDF00273 ((dimethylallyl)adenosine_tRNA)
   116C
   Binds [4Fe-4S] cluster
   80C, 116C, 151C
   SFLD
   SFLDF00273 ((dimethylallyl)adenosine_tRNA)
   151C
   Binds [4Fe-4S] cluster
   80C, 116C, 151C
   SFLD
   SFLDF00273 ((dimethylallyl)adenosine_tRNA)
2. SFLDG01082
   225C
   Binds [4Fe-4S]-AdoMet cluster
   225C, 229C, 232C
   SFLD
   SFLDG01082 (B12-binding_domain_containing)
   229C
   Binds [4Fe-4S]-AdoMet cluster
   225C, 229C, 232C
   SFLD
   SFLDG01082 (B12-binding_domain_containing)
   232C
   Binds [4Fe-4S]-AdoMet cluster
   225C, 229C, 232C
   SFLD
   SFLDG01082 (B12-binding_domain_containing)
3. SFLDG01061
   225C
   Binds [4Fe-4S]-AdoMet cluster
   225C, 229C, 232C
   SFLD
   SFLDG01061 (methylthiotransferase)
   229C
   Binds [4Fe-4S]-AdoMet cluster
   225C, 229C, 232C
   SFLD
   SFLDG01061 (methylthiotransferase)
   232C
   Binds [4Fe-4S]-AdoMet cluster
   225C, 229C, 232C
   SFLD
   SFLDG01061 (methylthiotransferase)
4. SFLDF00273
   225C
   Binds [4Fe-4S]-AdoMet cluster
   225C, 229C, 232C
   SFLD
   SFLDF00273 ((dimethylallyl)adenosine_tRNA)
   229C
   Binds [4Fe-4S]-AdoMet cluster
   225C, 229C, 232C
   SFLD
   SFLDF00273 ((dimethylallyl)adenosine_tRNA)
   232C
   Binds [4Fe-4S]-AdoMet cluster
   225C, 229C, 232C
   SFLD
   SFLDF00273 ((dimethylallyl)adenosine_tRNA)
5. SFLDF00413
   225C
   Binds [4Fe-4S]-AdoMet cluster
   225C, 229C, 232C
   SFLD
   SFLDF00413 (CDK5RAP1)
   229C
   Binds [4Fe-4S]-AdoMet cluster
   225C, 229C, 232C
   SFLD
   SFLDF00413 (CDK5RAP1)
   232C
   Binds [4Fe-4S]-AdoMet cluster
   225C, 229C, 232C
   SFLD
   SFLDF00413 (CDK5RAP1)

### GO term prediction

#### Biological Process

GO:0006400 tRNA modification

#### Molecular Function

GO:0003824 catalytic activity
GO:0016740 transferase activity
GO:0035596 methylthiotransferase activity
GO:0051536 iron-sulfur cluster binding
GO:0051539 4 iron, 4 sulfur cluster binding

#### Cellular Component

None predicted.

Generated with InterProScan 5.45-80.0