The following files were retrieved from NCBI https://www.ncbi.nlm.nih.gov/assembly/locust.OGS_preQC Information about this dataset can be found here: https://i5k.nal.usda.gov/bio_data/836515 Information about the functional annotation pipeline can be found here: https://www.mdpi.com/2075-4450/12/8/748 Instruction for running the functional annotation pipeline can be found here: https://agbase-docs.readthedocs.io/en/latest/agbase/workflow.html The file locust.OGS_preQC.gff3.pep was post-processed with agbase/goanna:2.2 (https://hub.docker.com/repository/docker/agbase/goanna) docker container to generate GO functional annotation. GOanna was run with these options: -a,invertebrates; -f,3; -g,70; -k,9; -q,70; -r,1.2. The resulting files are: locust.OGS_preQC.asn locust.OGS_preQC.gaf.tsv locust.OGS_preQC.html locust.OGS_preQC.tsv The file locust.OGS_preQC.gff3.pep was post-processed with agbase/interproscan:5.45-80_2 (https://hub.docker.com/repository/docker/agbase/interproscan) docker container to generate GO and pathway functional annotation. The resulting files are: locust.OGS_preQC_acc_go_counts.txt locust.OGS_preQC_acc_interpro_counts.txt locust.OGS_preQC_acc_pathway_counts.txt locust.OGS_preQC.err locust.OGS_preQC_gaf.txt locust.OGS_preQC.gff3 locust.OGS_preQC_go_counts.txt locust.OGS_preQC_interpro_counts.txt locust.OGS_preQC.json locust.OGS_preQC_pathway_counts.txt locust.OGS_preQC.tsv locust.OGS_preQC.xml locust.OGS_preQC.html.tar.gz locust.OGS_preQC.svg.tar.gz The file locust.OGS_preQC.gff3.pep was post-processed with agbase/kobas:3.0.3_0 (https://hub.docker.com/repository/docker/agbase/kobas) docker container to generate pathway functional annotation. The resulting files are: locust.OGS_preQC_KOBAS_acc_pathways.tsv locust.OGS_preQC_KOBAS_pathways_acc.tsv locust.OGS_preQC_KOBAS.txt The GAF output files from InterProScan and GOanna were combined into a single GAF file using agbase/combine_gafs:1.0 (https://hub.docker.com/repository/docker/agbase/combine_gafs) locust.OGS_preQC_complete.gaf.tsv