All wasps used in library construction were derived from an unmated female. They are all haploid and are haplodiploid siblings of each other. A short insert library was constructed from a single wasp (USDA ID 24.2). A 3 kb mate-paired library was constructed from a pool of individuals that are full siblings of the single wasp (USDA ID 24pool). An 8 kb mate-paired library was constructed from individuals that were half siblings of the single wasp (USDA ID 12pool). Sequencing and DNA extractions were done separately for each library then the reads from the three libraries were pooled to generate the assembly.
Additional information about this analysis:
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