- Data provider: Erin D. Scully (USDA-ARS-Grain, Forage, and Bioenergy Research Unit, Lincoln, NE), Scott M. Geib (USDA-ARS-Pacific Basin Agricultural Research Center, Hilo, HI), and Kelli Hoover (Pennsylvania State University, University Park, PA). - Bioproject accession number: PRJNA247974 - SRA accession number : SRR1290861 and SRR1292125 - Rearing conditions: Asian longhorned beetle adults were allowed to mate and oviposit onto potted sugar maple trees maintained at a USDA-approved quarantine greenhouse located at The Pennsylvania State University, University Park, PA. 60 days after the eggs hatched (indicated by the first appearance of frass), trees were harvested and third instar larvae were collected and surface sterilized with 70% ethanol. - Library preparation : Four whole larvae were homogenized and pooled together for RNA isolation. Total RNA was isolated from whole larvae using the Power Microbiome RNA isolation kit (MoBio, Carlsbad, CA) and ribosomal RNA was depleted from the sample using the Ribominus Eukaryotic Kit for RNA Seq (Life Technologies, Carlsbad, CA). The library was prepared using the TruSeq RNA Sample Prep Kit (Illumina, San Diego, CA) and the library was enriched for 175 nt fragments so that the paired ends overlapped by 30 nt. - Sequencing: Approximately 19 million 100 nt unstranded paired end reads were sequenced on the Illumina HiSeq 2000 platform in two separate runs. Data for runs 1 and 2 are available under the accession numbers SRR1290861 and SRR1292125, respectively. - Read cleaning method: Paired reads were quality filtered using ea-utils. Pairs where one or both reads had a mean quality score less of than 30 were discarded. - Alignment method: Remaining pairs were mapped to the ALB genome using TopHat2 with the following non-default parameters: --library-type fr-unstranded –mate-inner-dist -30 –mate-std-dev 50 –b2-sensitive.