Anoplophora glabripennis

Overview

The Asian long-horned beetle (Anoplophora glabripennis) (ALB) is an invasive pest from Asia that came to Canada, the United States and Europe concealed in solid wood packing material.

It is a serious threat to deciduous hardwood trees in urban, suburban, and forested parts of the country. Larvae bore into a tree's heartwood, damaging and eventually killing the tree. If it became widely established in North America (it is already established locally), it could be one of the most destructive and costly invasive species ever (USDA Program Aid No.1655). This is target of current USDA eradication efforts.

These beetles are large (1-1.5 inches), and one beetle can provide more than 10 micrograms of DNA. Specimens in North America are all relatively closely related. Suitable specimens are available for sequencing.

All files were generated by the Baylor College of Medicine's i5k pilot project. The original source for these files is here. Please cite the following publication when using the A. glabripennis genome and annotations:
McKenna, D. D., Scully, E. D., Pauchet, Y., et al. Genome of the Asian longhorned beetle (Anoplophora glabripennis), a globally significant invasive species, reveals key functional and evolutionary innovations at the beetle–plant interface. Genome Biology 2017 17(1), 227. DOI: 10.1186/s13059-016-1088-8

Community contact:

Image Credit: Appleby James, U.S. Fish and Wildlife Service. View Source.
Organism Image
Feature Summary
The following features are currently present for this organism
Feature TypeCount
CDS99,911
mRNA22,037
Gene22,035
Contig N50
16551
Scaffold N50
658851
Number Of Genes
21004
Community Contact
Community Contact: 
Duane McKenna|dmckenna@memphis.edu
Image Credit
Appleby James, U.S. Fish and Wildlife Service. <a href="http://www.public-domain-image.com/free-images/fauna-animals/insects-and-bugs/beetle-insects-pictures/asian-longhorn-beetle-anoplophora-glabripennis-invasive-species">View Source.</a>

Assembly Information

Analysis Name Whole genome assembly of Anoplophora glabripennis
Software Baylor College of Medicine genome assembly pipeline (NA)
Source Anoplophora glabripennis Bt103082013 assembly
Date performed 2013-07-16
Materials & Methods

Sequence generation for assembly. For this project we are generating fairly high coverage in a number of different insert sized libraries. The assembly strategy is based around a seed allpaths assembly (the Broad Allpaths assembler) followed by seed assembly improvement using homegrown tools, Atlas-link and Atlas-GapFill, which can significantly improve the results. Thus we generate sequence data to enable the Allpaths assembly. As of Nov 2011 this is: - 40X genome coverage in 180bp insert library (100bp reads forward and reverse); and 40X 3kb insert data. To enable better scaffolding and local gap filling we additionally generate 500bp, 1kb, 2kb, and 8kb insert sizes at > 20X coverage.


Source: Baylor College of Medicine i5K Project Summary

Statistics

Assembly Metrics
Contig N50 16551
Scaffold N50 658851
GC Content 32.47