The mayfly Ephemera danica occurs in Central Europe in creeks and rivers with a high water quality. Its larvae feed on detritus. Its adults do not ingest any more and only live 2-4 days. During this time, the adult mayflies have to find a mating partner, mate, and deposit eggs.
Ephemeroptera are considered as one of the earliest lineages of flying insects. As such, mayflies play an important role for understanding the evolutionary roots of flight, one of the most important and dramatic steps in the evolutionary history of insects. Despite their highly interesting systematic position, genomic data of mayflies are scarce. The i5K initiative therefore decided to sequence the genome of a mayfly.
Data were generated by the Baylor College of Medicine's i5k pilot project.
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The following features are currently present for this organism
Number Of Genes
<a href="https://commons.wikimedia.org/wiki/User:Jeffdelonge">Jeffdelonge.</a> <a href="https://commons.wikimedia.org/wiki/File:Ephemera_danica01.jpg">View Source.</a> <a href="https://creativecommons.org/licenses/by-sa/3.0/deed.en">CC-BY-SA-3.0</a>
|Analysis Name||Whole genome assembly of Ephemera danica|
|Software||Baylor College of Medicine genome assembly pipeline (NA)|
|Source||Ephemera danica genome assembly Edan07162013.scaffolds|
|Materials & Methods||
Sequence generation for assembly. For this project we are generating fairly high coverage in a number of different insert sized libraries. The assembly strategy is based around a seed allpaths assembly (the Broad Allpaths assembler) followed by seed assembly improvement using homegrown tools, Atlas-link and Atlas-GapFill, which can significantly improve the results. Thus we generate sequence data to enable the Allpaths assembly. As of Nov 2011 this is: - 40X genome coverage in 180bp insert library (100bp reads forward and reverse); and 40X 3kb insert data. To enable better scaffolding and local gap filling we additionally generate 500bp, 1kb, 2kb, and 8kb insert sizes at > 20X coverage.
Source: Baylor College of Medicine i5K Project Summary