Eurytemora affinis

Overview

Copepods form the largest biomass of metazoans in the world’s oceans, and dominate zooplankton assemblages in nearshore environments. In particular, the copepod Eurytemora affinis has an enormous biomass (10^4-10^5/m3) in many coastal systems worldwide, including the Gulf of Mexico, St. Lawrence, Chesapeake Bay, Columbia River estuary, Baltic Sea, and estuaries of Europe. This copepod is a dominant grazer of algae and major food source for some of the world's most important fisheries, such as herring, anchovy, salmon, and flounder. Given the numerical dominance of E. affinis in coastal waters, with estimated census sizes in the billions, this copepod has significant impacts on coastal ecosystems.

Moreover, there has been great interest in E. affinis as a waterborne disease vector. Copepods harbor an enormous biomass in their microbiomes. Our sequencing of the E. affinis microbiome has uncovered several putatively pathogenic taxa (not present in the surrounding water), including Vibrio cholerae, Salmonella, Shigella, Campylobacter, Corynebacterium diphtheriae, Yersinia, Aeromonas hydrophila, and Acinetobacter haemolyticus.

E. affinis is an invasive species, which moves readily from coastal habitats into inland waters, such that the composition of its microbial community could have serious implications for disease transmission. E. affinis exhibits rapid physiological evolution during invasions from coastal into inland waters, as well as shifts in its microbiome during invasions. Thus, E. affinis provides a valuable model for studying evolution during biological invasions as well as the transport of pathogens by invasive hosts. Waterborne diseases are poorly understood, and E. affinis provides the opportunity to study pathways and mechanisms of waterborne disease transmission.(1)

Data were generated by the Baylor College of Medicine's i5k pilot project.

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(1) Eurytemora affinis. arthropodgenomes.org. 7 June 2012.

Community contact:

Image Credit: Copyright Dr. Carol Eunmi Lee View Source.
Organism Image
Feature Summary
The following features are currently present for this organism
Feature TypeCount
CDS172,972
mRNA29,783
Gene29,783
Contig N50
5738
Scaffold N50
862645
Number Of Genes
29783
Community Contact
Community Contact: 
Carol Lee|carollee@wisc.edu
Image Credit
Copyright Dr. Carol Eunmi Lee <a href="http://life.bio.sunysb.edu/marinebio/plankton.html">View Source.</a>

Assembly Information

Analysis Name Whole genome assembly of Eurytemora affinis
Software Baylor College of Medicine genome assembly pipeline (NA)
Source Eurytemora affinis genome assembly Eaff_11172013.genome.fa
Date performed 2013-11-17
Materials & Methods

Sequence generation for assembly. For this project we are generating fairly high coverage in a number of different insert sized libraries. The assembly strategy is based around a seed allpaths assembly (the Broad Allpaths assembler) followed by seed assembly improvement using homegrown tools, Atlas-link and Atlas-GapFill, which can significantly improve the results. Thus we generate sequence data to enable the Allpaths assembly. As of Nov 2011 this is: - 40X genome coverage in 180bp insert library (100bp reads forward and reverse); and 40X 3kb insert data. To enable better scaffolding and local gap filling we additionally generate 500bp, 1kb, 2kb, and 8kb insert sizes at > 20X coverage.


Source: Baylor College of Medicine i5K Project Summary

Statistics

Assembly Metrics
Contig N50 5738
Scaffold N50 862645
GC Content 32.48
Manual Annotations