Bactrocera oleae genome assembly MU_Boleae_v2 (GCF_001188975.3)

We have sequenced the olive fruit fly (Bactrocera oleae) with Illumina short reads, Illumina mate pairs, PacBio long reads and have also performed a de novo transcriptome assembly with Illumina RNA sequencing.

The total genome size we have obtained is 512,888,968 bp in 77,416 sequences. By combining all the technologies starting with the initial short read assembly and using Illumina mate pairs we were able to increase the GN50 from 9,039 bp in 9,547 sequences to 113,040 bp in 608 sequences. This scaffolded assembly was then upgraded to a GN50 of 205,997 bp in 373 sequences using a PacBio continuous long reads and submitted to NCBI as GenBank: LGAM00000000.1

Finally we have used reassembled transcripts to further increase the size of the scaffolded assembly to a GN50 of 250,814 bp in 324 sequences.

The work represents a collaboration between Greek and Canadian Teams lead by Drs Kostas Mathiopoulos and Jiannis Ragoussis respectively. The University of Thessaly team in Greece consists of Kostas Mathiopoulos, Konstantina Tsoumani, Maria-Eleni Gregoriou, Efthimia Sagri. The BSRC Fleming team in Athens, Greece includes Martin Reczko and Jiannis Ragoussis (Affiliated Member). The McGill University and Genome Quebec Innovation Centre team in Montreal includes Jiannis Ragoussis, Haig Djambazian, Ken Dewar, and Louis Letourneau. The work is supported by the Action “ARISTEIA” of the “Operational program “Education and Life Long Learning”, co-funded by the European Social Fund and Greek National Resources”; and Genome Canada.