Leptinotarsa decemlineata

Overview

The Colorado potato beetle originated from the Mexican highlands, and is now considered the economically most significant defoliator of potato in northern latitudes worldwide. The range of this insect is continuing to expand, and it is likely to eventually colonize all potato-producing areas with temperate climate. Within Mexico, the beetle feeds on native solanaceous plants, S. angustifolium, S. elaeagnifolium, and buffalo bur, S. rostratum. However, it has adapted to potatoes and other solanaceous crops after its range expansion.

Due to the lack of any natural enemies that have been able to evolve seasonal adaptations, the cornerstone of Colorado potato beetle management has been the use of insecticides. However, the beetle has shown a remarkable ability to develop resistance to most insecticides used for its control. The mechanism(s) of insecticide resistance is yet unknown and genomic sequencing will lead to major advances in managing this pest in commercial plant production.

Data were generated by the Baylor College of Medicine's i5k pilot project.

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Community contact:

Image Credit: Scott Bauer, USDA Agricultural Research Service, Bugwood.org. CCBY license. View Source.

Organism Image
Feature Summary
The following features are currently present for this organism
Feature TypeCount
CDS91,578
mRNA24,671
Gene24,671
Contig N50
4137
Scaffold N50
361260
Number Of Genes
24671
Community Contact
Community Contact: 
Sean Schoville|schoville2@wisc.edu
Yolanda Chen|Yolanda.Chen@uvm.edu
Image Credit
Scott Bauer, USDA Agricultural Research Service, Bugwood.org. <a href="http://creativecommons.org/licenses/by/3.0/">CCBY license.</a> <a href="http://www.insectimages.org/browse/detail.cfm?imgnum=1321016">View Source.</a></p>

Assembly Information

Analysis Name Whole genome assembly of Leptinotarsa decemlineata
Software Baylor College of Medicine genome assembly pipeline (NA)
Source Leptinotarsa decemlineata Ldec assembly
Date performed 2013-09-18
Materials & Methods

Sequence generation for assembly. For this project we are generating fairly high coverage in a number of different insert sized libraries. The assembly strategy is based around a seed allpaths assembly (the Broad Allpaths assembler) followed by seed assembly improvement using homegrown tools, Atlas-link and Atlas-GapFill, which can significantly improve the results. Thus we generate sequence data to enable the Allpaths assembly. As of Nov 2011 this is: - 40X genome coverage in 180bp insert library (100bp reads forward and reverse); and 40X 3kb insert data. To enable better scaffolding and local gap filling we additionally generate 500bp, 1kb, 2kb, and 8kb insert sizes at > 20X coverage.


Source: Baylor College of Medicine i5K Project Summary

Statistics

Assembly Metrics
Contig N50 4137
Scaffold N50 361260
GC Content 36.03
Manual Annotations