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i5k Workspace@NAL
U.S. Department of Agriculture
  1. Organism
  2. Oncopeltus Fasciatus

Oncopeltus fasciatus

Summary
Type
organism
Genus
Oncopeltus
Species
fasciatus
Common Name
Milkweed bug
Description

Oncopeltus fasciatus has been an established lab organism for over 60 years, and has been used for a wide range of studies from physiology to development and evolution. As a relatively conservative and generalized species, it affords a baseline against which other species can be compared.

For example, this species has the same piercing and sucking type mouthparts as its less benign relatives, including the blood-sucking kissing bug, Rhodnius prolixus, and the brown marmorated stink bug, Halyomorpha halys, which are disease vector and agricultural pest species, respectively. Unlike the pest species, the benign, seed-feeding Oncopeltus can be functionally investigated in the lab by RNA interference (RNAi). Comparing the genomes, and conducting experimental lab work in Oncopeltus, will help to identify unique features of the pest species, and thus inform management strategies for them.

More generally, Oncopeltus is a key species for comparisons across the insects. It is one of the few experimentally tractable hemimetabolous species that can ground comparisons with the completely metamorphosing species of the Holometabola (e.g., flies, beetles, wasps). Topics investigated in this framework include reproductive biology and development of the legs, wings, body segments, extraembryonic membranes, and overall establishment of the body plan.

Data were generated by the Baylor College of Medicine's i5k pilot project(link is external).

View the Baylor College of Medicine's data sharing policy(link is external).

Please cite the following publications when using the genome assembly Ofas_1.0, annotations BCMv0.5.3, or annotations OGSv1.1:

  • Genome assembly Ofas_1.0: (dataset) Murali, Shwetha C.; Bandaranaike, D.; Bellair, M.; et al. (2015). Oncopeltus fasciatus genome assembly 1.0. Ag Data Commons. http://dx.doi.org/10.15482/USDA.ADC/1173238
  • Genome annotations BCMv0.5.3:(dataset) Hughes, D. S. T.; Koelzer, S.; Panfilio, K. A.; Richards, Stephen (2015). Oncopeltus fasciatus genome annotations v0.5.3. Ag Data Commons. http://dx.doi.org/10.15482/USDA.ADC/1173237
  • Genome annotations OGSv1.2:(dataset) Vargas Jentzsch, Iris M.; Hughes, Daniel S. T.; Poelchau, Monica; et al. (2015). Oncopeltus fasciatus Official Gene set v1.2. Ag Data Commons. https://doi.org/10.15482/USDA.ADC/1518752
Publication
  • Panfilio KA, Vargas Jentzsch IM, Benoit JB, Erezyilmaz D, Suzuki Y, Colella S, Robertson HM, Poelchau MF, Waterhouse RM, Ioannidis P, Weirauch MT, Hughes DST, Murali SC, Werren JH, Jacobs CGC, Duncan EJ, Armisén D, Vreede BMI, Baa-Puyoulet P, Berger CS, Chang CC, Chao H, Chen MM, Chen YT, Childers CP, Chipman AD, Cridge AG, Crumière AJJ, Dearden PK, Didion EM, Dinh H, Doddapaneni HV, Dolan A, Dugan S, Extavour CG, Febvay G, Friedrich M, Ginzburg N, Han Y, Heger P, Holmes CJ, Horn T, Hsiao YM, Jennings EC, Johnston JS, Jones TE, Jones JW, Khila A, Koelzer S, Kovacova V, Leask M, Lee SL, Lee CY, Lovegrove MR, Lu HL, Lu Y, Moore PJ, Munoz-Torres MC, Muzny DM, Palli SR, Parisot N, Pick L, Porter ML, Qu J, Refki PN, Richter R, Rivera-Pomar R, Rosendale AJ, Roth S, Sachs L, Santos ME, Seibert J, Sghaier E, Shukla JN, Stancliffe RJ, Tidswell O, Traverso L, van der Zee M, Viala S, Worley KC, Zdobnov EM, Gibbs RA, Richards S. Molecular evolutionary trends and feeding ecology diversification in the Hemiptera, anchored by the milkweed bug genome. . Genome biology. 2019 04 02; 20(1):64.
  • Thomas GWC, Dohmen E, Hughes DST, Murali SC, Poelchau M, Glastad K, Anstead CA, Ayoub NA, Batterham P, Bellair M, Binford GJ, Chao H, Chen YH, Childers C, Dinh H, Doddapaneni HV, Duan JJ, Dugan S, Esposito LA, Friedrich M, Garb J, Gasser RB, Goodisman MAD, Gundersen-Rindal DE, Han Y, Handler AM, Hatakeyama M, Hering L, Hunter WB, Ioannidis P, Jayaseelan JC, Kalra D, Khila A, Korhonen PK, Lee CE, Lee SL, Li Y, Lindsey ARI, Mayer G, McGregor AP, McKenna DD, Misof B, Munidasa M, Munoz-Torres M, Muzny DM, Niehuis O, Osuji-Lacy N, Palli SR, Panfilio KA, Pechmann M, Perry T, Peters RS, Poynton HC, Prpic NM, Qu J, Rotenberg D, Schal C, Schoville SD, Scully ED, Skinner E, Sloan DB, Stouthamer R, Strand MR, Szucsich NU, Wijeratne A, Young ND, Zattara EE, Benoit JB, Zdobnov EM, Pfrender ME, Hackett KJ, Werren JH, Worley KC, Gibbs RA, Chipman AD, Waterhouse RM, Bornberg-Bauer E, Hahn MW, Richards S. Gene content evolution in the arthropods. . Genome biology. 2020 01 23; 21(1):15.
Organism Image
Image Credit
Copyright Kristen Panfilio. View Source.(link is external)
Assembly Stats
Contig N50
4047
GC Content
32.62
Scaffold N50
339960
Other Information
Community Contact
Kristen Panfilio, University of Hohenheim; Ariel Chipman, The Hebrew University of Jerusalem
Links
Oncopeltus fasciatus @ Baylor College of Medicine(link is external)
NCBI BioProject(link is external)
Download Data
Analyses
Name Program
BCM annotation of the Oncopeltus fasciatus assembly using Maker and additional analyses MAKER
Oncopeltus fasciatus Official Gene Set v1.1 BCM created initial set; Panfilio refined and released final OGS
Ginzburg et al. embryo RNA-Seq FastQC, trimomatic, MultiQC, STARaligner
Whole genome assembly of Oncopeltus fasciatus Baylor College of Medicine genome assembly pipeline
Oncopeltus fasciatus Official Gene Set v1.2 https://github.com/NAL-i5K/I5KNAL_OGS
Oncopeltus fasciatus genome assembly Ofas_1.0 (GCA_000696205.1) AllPaths LG v. 44620; Atlas Link v. 1.0; Atlas GapFill v. 2.2
Functional annotation of Oncopeltus fasciatus Official Gene Set v1.2 AgBase functional annotation pipeline
Oncopeltus fasciatus annotations GCA_000696205.1_Ofas_1.0 MAKER2, manual annotation, GFF3toolkit