Fopius arisanus

Overview

Fopius arisanus (Sonan, 1932) is an egg-pupal parasitoid of Tephritid fruit flies. This Braconid wasp has been utilized for biological control of the Mediterranean fruit fly (Ceratitis capitata), oriental fruit fly (Bactrocera dorsalis), as well as many other Tephritid species, which are economic pests world-wide. F. arisanus lays its eggs into the egg of the host fly, and develop during egg and larvae stages to emerge from pupa of the host as an adult wasp. Foundational genomic and transcriptomic information in this species serve as a tool to help in understanding the underlying mechanisms that contribute to parasitism behavior, describing the physiology and biology of host selection and host/parasitoid interactions, the design of better biological control strategies, and the development of monitoring tools for parasitism rate in the field. In addition, to our knowledge, this is the first member of the Opiinae to have significant molecular data collected from it, which includes many important parasitoids used for biological control (e.g. Diachasmimorpha sp. and Psyttalia sp.) and can be used in comparative genomics with other sequenced hymenopterans. Like other hymenopterans, this species is haplodiploid, with females being diploid and males haploid. To generate this assembly, we pooled haploid males from a single unmated female to reduce heterozygosity of the assembly, essentially representing the genome of the parental female.

Image author and copyright: Amy Carmichael, Queensland University of Technology, Image source (Creative Commons Attribution 3.0 Australia License)
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Organism Image
Feature Summary
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Contig N50
NA
Scaffold N50
978588
Number Of Genes
11805
Community Contact
Community Contact: 
Scott Geib, USDA-ARS|Scott.geib@ars.usda.gov

Assembly Information

Analysis Name Fopius arisanus genome assembly Fari_v1
Software AllPaths (49856)
Source Fopius arisanus strain USDA-PBARC FA_bdor, whole genome shotgun sequencing project
Date performed 2015-02-22
Materials & Methods All wasps used in library construction were derived from an unmated female. They are all haploid and are haplodiploid siblings of each other. A short insert library was constructed from a single wasp (USDA ID 24.2). A 3 kb mate-paired library was constructed from a pool of individuals that are full siblings of the single wasp (USDA ID 24pool). An 8 kb mate-paired library was constructed from individuals that were half siblings of the single wasp (USDA ID 12pool). Sequencing and DNA extractions were done separately for each library then the reads from the three libraries were pooled to generate the assembly.

Statistics

Assembly Metrics
Contig N50 NA
Scaffold N50 978588
GC Content 39.44
Manual Annotations