Table of Contents
The japygid Catajapyx aquilonaris is a blind predator of the soil. Like Protura (Acerentomon maius) and Collembola (Sminthurus viridis), Diplura lack wings, mirroring the wingless insect ancestor. Like in all primarily wingless hexapods, sperms are not transferred directly during copulation. Males rather deposit a spermatophore on the ground and females subsequently take the spermatophore up.
Diplura are critical for understanding the evolutionary origin of Hexapoda (e.g., terrestrialization), the evolutionary origin of wings (ancestral condition in Diplura), and the evolution of direct sperm transfer (ancestral condition in Diplura).
Data were generated by the Baylor College of Medicine's i5k pilot project.
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- Analysis Name
- Whole genome assembly of Catajapyx aquilonaris
- Baylor College of Medicine genome assembly pipeline (NA)
- Date performed
- Materials & Methods
Sequence generation for assembly. For this project we are generating fairly high coverage in a number of different insert sized libraries. The assembly strategy is based around a seed allpaths assembly (the Broad Allpaths assembler) followed by seed assembly improvement using homegrown tools, Atlas-link and Atlas-GapFill, which can significantly improve the results. Thus we generate sequence data to enable the Allpaths assembly. As of Nov 2011 this is: - 40X genome coverage in 180bp insert library (100bp reads forward and reverse); and 40X 3kb insert data. To enable better scaffolding and local gap filling we additionally generate 500bp, 1kb, 2kb, and 8kb insert sizes at > 20X coverage.
Source: Baylor College of Medicine i5K Project Summary
- Contig N50
- Scaffold N50
- GC Content