Frankliniella occidentalis

Overview

Frankliniella occidentalis, the western flower thrips, is a representative of the order Thysanoptera. This insect order is composed exclusively of tiny insects commonly called thrips, and includes more than 7,000 species.

Thrips are considered to be members of the hemipteroid assemblage, which includes the orders Hemiptera, Pscocoptera, and Phthiraptera.

Thysanopterans display an extraordinary amount of diversity with respect to food preference and behaviors. They share, however, some common, but unique, characteristics such as asymmetrical mouthparts composed of a fully developed left mandible and a resorbed right mandible during embryonic development and postembryonic ‘remetaboly’ development, a process that is intermediate between holometaboly and hemimetaboly development.

Frankliniella occidentalis is the most economically-important agronomic pest worldwide within Thysanoptera because it is a direct pest and an efficient vector of plant pathogenic viruses. F. occidentalis is polyphagous, feeding on a wide array of plant species and plant organs.

The insect is haplo-diploid, i.e., haploid males arise from unfertilized eggs, while diploid females develop from fertilized eggs. The short reproductive cycle (~12 days, egg to adult) and high fecundity (~75 eggs per female) of this species contributes to its success as an invasive species.

This species transmits at least five described Tospovirus species (negative strand RNA viruses) to hundreds of food, fiber, and ornamental crops. F. occidentalis efficiently transmits the type member, Tomato spotted wilt virus (TSWV), in a persistent propagative fashion and the virus is passed transstadially from the young larval to pupul to adult developmental stages.

Despite their diversity, biological uniqueness, and economic importance as world-wide crop pests, there are currently no genomics resources available for insects classified in Thysanoptera. Genomics resources for this thrips species would provide tools to identify vector proteins involved in virus acquisition, replication and movement inside thrips tissues, and transmission to plants.

Data were generated by the Baylor College of Medicine's i5k pilot project.

View the Baylor College of Medicine's data sharing policy.

Community contact:

Image Credit: Whitney Cranshaw, Colorado State Univeristy. CCBY license. View Source
Organism Image
Feature Summary
The following features are currently present for this organism
Feature TypeCount
CDS105,852
mRNA17,553
Gene17,553
Contig N50
6159
Scaffold N50
948890
Number Of Genes
17553
Community Contact
Community Contact: 
Dorith Rotenberg|drotenbe@k-state.edu
Image Credit
Whitney Cranshaw, Colorado State Univeristy. <a href="http://creativecommons.org/licenses/by/3.0/">CCBY license.</a> <a href="http://www.insectimages.org/browse/detail.cfm?imgnum=1476101">View Source</a>

Assembly Information

Analysis Name Whole genome assembly of Frankliniella occidentalis
Software Baylor College of Medicine genome assembly pipeline (NA)
Source Frankliniella occidentalis genome assembly Focc_FINAL.scaffolds.fa
Date performed 2013-12-06
Materials & Methods

Sequence generation for assembly. For this project we are generating fairly high coverage in a number of different insert sized libraries. The assembly strategy is based around a seed allpaths assembly (the Broad Allpaths assembler) followed by seed assembly improvement using homegrown tools, Atlas-link and Atlas-GapFill, which can significantly improve the results. Thus we generate sequence data to enable the Allpaths assembly. As of Nov 2011 this is: - 40X genome coverage in 180bp insert library (100bp reads forward and reverse); and 40X 3kb insert data. To enable better scaffolding and local gap filling we additionally generate 500bp, 1kb, 2kb, and 8kb insert sizes at > 20X coverage.


Source: Baylor College of Medicine i5K Project Summary

Statistics

Assembly Metrics
Contig N50 6159
Scaffold N50 948890
GC Content 54.28
Manual Annotations