Table of Contents
Copidosoma floridanum is the best studied insect that undergoes polyembryonic development. Its development is highly novel and has also evolved a caste (social) system by novel mechanism.
Particularly unique is this species produces >2000 clonal progeny per egg that are genetically identical. This unique feature of development provides an enormous quantity of DNA for full genome sequencing that derives from a single male (haploid) egg. The technical advantage this provides is unrivaled by any other parasitic hymenopteran.
The species is in continuous culture such that material is immediately available. The species is important to agriculture as the wasp is used in biological control. The species is also phylogenitically well placed for comparison to other species like Nasonia and the honeybee.
Data were generated by the Baylor College of Medicine's i5k pilot project.
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- Analysis Name
- Whole genome assembly of Copidosoma floridanum
- Baylor College of Medicine genome assembly pipeline (NA)
- Copidosoma floridanum genome assembly Cflo.scaffolds.fa
- Date performed
- Materials & Methods
Sequence generation for assembly. For this project we are generating fairly high coverage in a number of different insert sized libraries. The assembly strategy is based around a seed allpaths assembly (the Broad Allpaths assembler) followed by seed assembly improvement using homegrown tools, Atlas-link and Atlas-GapFill, which can significantly improve the results. Thus we generate sequence data to enable the Allpaths assembly. As of Nov 2011 this is: - 40X genome coverage in 180bp insert library (100bp reads forward and reverse); and 40X 3kb insert data. To enable better scaffolding and local gap filling we additionally generate 500bp, 1kb, 2kb, and 8kb insert sizes at > 20X coverage.
Source: Baylor College of Medicine i5K Project Summary
- Contig N50
- Scaffold N50
- GC Content