Manual annotation and Apollo

Manual annotation learning and training resources

We’ve put together resources to help you learn more about manual annotation, in particular via the Apollo software – whether you’re new to manual annotation, are looking to train undergraduates in manual annotation, or just need more information.

What is manual annotation, and how does Apollo help me do it? 

There are a number of definitions of manual annotation. Within the Apollo context, it means the manual improvement of a gene prediction – both the structural (e.g. the protein and transcript sequence) and functional (e.g. the name and other metadata) components. Apollo is a software package that greatly simplifies the manual annotation process via a user-friendly web interface. The i5k Workspace@NAL provides the Apollo software for all of the genome projects that we host. 

How do I get an account to annotate in Apollo? 

You can register for an i5k Workspace Apollo account here:

New to manual annotation and Apollo, or need a refresher? We recommend the following tutorials:

Working with undergraduates? 

Need a deeper dive?

Other software

The i5k Workspace@NAL offers manual annotation resources via Apollo. Feel free to get in touch with us if you need Apollo set up for your genome! That said, our resources don’t always fit everyone’s needs. If you need to branch out, here are some resources that may be useful to you: 

Do you have suggestions for improving this list of resources? Contact us – we love feedback.