Table of Contents
Limnephilus lunatus is a common species and a typical case-bearing caddisfly. Like almost all Trichoptera, this species has an aquatic larva. The adults are moth-like and can be found in the vicinity of the waters, in which their larvae develop. Many caddisfly species play an important role as bioindicators in limnological studies. The phylogeny and evolution of the caddisfies is, however, still poorly understood. Together with Lepidoptera (moths and butterflies), Trichoptera form a well-supported clade within holometabolous insects called Amphiesmenoptera.
Trichoptera are important to understand the evolution of heterogametic females and of the biosynthesis of silk, both features Trichoptera share with Lepidoptera. Trichoptera are further important as outgroup to better understand the evolution of Lepidoptera.
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- Analysis Name
- Whole genome assembly of Limnephilus lunatus
- Baylor College of Medicine genome assembly pipeline (NA)
- Limnephilus lunatus Llun assembly
- Date performed
- Materials & Methods
Sequence generation for assembly. For this project we are generating fairly high coverage in a number of different insert sized libraries. The assembly strategy is based around a seed allpaths assembly (the Broad Allpaths assembler) followed by seed assembly improvement using homegrown tools, Atlas-link and Atlas-GapFill, which can significantly improve the results. Thus we generate sequence data to enable the Allpaths assembly. As of Nov 2011 this is: - 40X genome coverage in 180bp insert library (100bp reads forward and reverse); and 40X 3kb insert data. To enable better scaffolding and local gap filling we additionally generate 500bp, 1kb, 2kb, and 8kb insert sizes at > 20X coverage.
Source: Baylor College of Medicine i5K Project Summary
- Contig N50
- Scaffold N50
- GC Content