Loxosceles reclusa

Overview
Potentially lethal toxin. Model organism for understanding dermonecrotic envenomation (sphingomylinase D). Focus of research to understand neurotic fractions in venom. Provides appropriate phylogenetic sampling across diversity of order Araneae. Easily obtained from colleagues in Oregon.

Data were generated by the Baylor College of Medicine's i5k pilot project.

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Community contact:

Organism Image
Feature Summary
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Contig N50
1834
Scaffold N50
63233
Number Of Genes
20617
Community Contact
Community Contact: 
Greta Binford|binford@lclark.edu
Image Credit
<a href="https://commons.wikimedia.org/wiki/User:Rosa_Pinedas">Rosa Pineda.</a> <a href="https://commons.wikimedia.org/wiki/File:Brown_Recluse.jpg">View Source.</a> <a href="https://creativecommons.org/licenses/by-sa/3.0/deed.en">CC-BY-SA-3.0</a>

Assembly Information

Analysis Name Whole genome assembly of Loxosceles reclusa
Software Baylor College of Medicine genome assembly pipeline (NA)
Source Lrec.scaffolds.fa
Date performed 2014-01-29
Materials & Methods

Sequence generation for assembly. For this project we are generating fairly high coverage in a number of different insert sized libraries. The assembly strategy is based around a seed allpaths assembly (the Broad Allpaths assembler) followed by seed assembly improvement using homegrown tools, Atlas-link and Atlas-GapFill, which can significantly improve the results. Thus we generate sequence data to enable the Allpaths assembly. As of Nov 2011 this is: - 40X genome coverage in 180bp insert library (100bp reads forward and reverse); and 40X 3kb insert data. To enable better scaffolding and local gap filling we additionally generate 500bp, 1kb, 2kb, and 8kb insert sizes at > 20X coverage.


Source: Baylor College of Medicine i5K Project Summary

Statistics

Assembly Metrics
Contig N50 1834
Scaffold N50 63233
GC Content 39.10
Manual Annotations