Table of Contents
This spider is the primary chelicerate model for evo/devo research in a growing number of labs worldwide. It is easy to culture, and a range of functional tools have been developed, including in situ hybridisation, embryonic and parental RNAi, and transient transformation. A number of transcriptome sequencing projects have been established.
As well as a powerful model for the evolution of development, this species has great potential for understanding other features of spider biology, including venom and silk production.
Data were generated by the Baylor College of Medicine's i5k pilot project.
If you use the data from this project in your research, please cite the following publication:
Schwager, E.E. et al. 2017. The house spider genome reveals an ancient whole-genome duplication during arachnid evolution. BMC Biology 15:62. https://doi.org/10.1186/s12915-017-0399-x
- Analysis Name
- Whole genome assembly of Parasteatoda tepidariorum
- Baylor College of Medicine genome assembly pipeline (NA)
- Parasteatoda tepidariorum Ptep01282013 assembly
- Date performed
- Materials & Methods
Sequence generation for assembly. For this project we are generating fairly high coverage in a number of different insert sized libraries. The assembly strategy is based around a seed allpaths assembly (the Broad Allpaths assembler) followed by seed assembly improvement using homegrown tools, Atlas-link and Atlas-GapFill, which can significantly improve the results. Thus we generate sequence data to enable the Allpaths assembly. As of Nov 2011 this is: - 40X genome coverage in 180bp insert library (100bp reads forward and reverse); and 40X 3kb insert data. To enable better scaffolding and local gap filling we additionally generate 500bp, 1kb, 2kb, and 8kb insert sizes at > 20X coverage.Source: Baylor College of Medicine i5K Project Summary
- Contig N50
- Scaffold N50
- GC Content