Parasteatoda tepidariorum

Overview

This spider is the primary chelicerate model for evo/devo research in a growing number of labs worldwide. It is easy to culture, and a range of functional tools have been developed, including in situ hybridisation, embryonic and parental RNAi, and transient transformation. A number of transcriptome sequencing projects have been established.

As well as a powerful model for the evolution of development, this species has great potential for understanding other features of spider biology, including venom and silk production.

Data were generated by the Baylor College of Medicine's i5k pilot project.

View the Baylor College of Medicine's data sharing policy.

Community contact:

Organism Image
Feature Summary
The following features are currently present for this organism
Feature TypeCount
CDS164,137
mRNA30,041
Gene30,041
Contig N50
10149
Scaffold N50
465572
Number Of Genes
30041
Community Contact
Community Contact: 
Alistair McGregor|amcgregor@brookes.ac.uk
Image Credit
<a href="https://en.wikipedia.org/wiki/User:Patrick0Moran">Patrick0Moran.</a> <a href="https://commons.wikimedia.org/wiki/File:Achae_tepid.JPG">View Source.</a> <a href="https://creativecommons.org/licenses/by-sa/3.0/deed.en">CC-BY-SA-3.0</a>

Assembly Information

Analysis Name Whole genome assembly of Parasteatoda tepidariorum
Software Baylor College of Medicine genome assembly pipeline (NA)
Source Parasteatoda tepidariorum Ptep01282013 assembly
Date performed 2013-07-16
Materials & Methods

Sequence generation for assembly. For this project we are generating fairly high coverage in a number of different insert sized libraries. The assembly strategy is based around a seed allpaths assembly (the Broad Allpaths assembler) followed by seed assembly improvement using homegrown tools, Atlas-link and Atlas-GapFill, which can significantly improve the results. Thus we generate sequence data to enable the Allpaths assembly. As of Nov 2011 this is: - 40X genome coverage in 180bp insert library (100bp reads forward and reverse); and 40X 3kb insert data. To enable better scaffolding and local gap filling we additionally generate 500bp, 1kb, 2kb, and 8kb insert sizes at > 20X coverage.


Source: Baylor College of Medicine i5K Project Summary

Statistics

Assembly Metrics
Contig N50 10149
Scaffold N50 465572
GC Content 29.83