Trichogramma pretiosum

Overview

Trichogramma pretiosum is a tiny egg parasitoid that is released by the millions as a biological control agent in Central and South America and to a lesser extent in North America and Asia. It is under consideration for the control of Tomato pests in Europe. It occurs naturally in North and South America. It has become established in Australia and on islands in the Pacific.

Several other Trichogramma species are also used in biological control and these are closely related to T. pretiosum. T. pretiosum has a short lifecyle of 11 days at 25 degrees Celsius and can be maintained on the eggs of the grainmoth Ephesia kuehniella.

Eggs of this moth species can be acquired through commercial suppliers. Because of their small size many different cultures can be maintained in a small space, we maintain our Trichogramma cultures in glass vials of 10cm*1.5cm.

In addition to its importance in biological control of lepidopteran pests, it is also very interesting because some populations are completely asexual because of an infection with parthenogenesis-inducing Wolbachia. The age of these completely infected populations varies and their prolonged asexual reproduction has led to a decay in traits involved in sexual reproduction. For instance, in the asexual populations from Peru, females have lost the ability to fertilize their eggs, while males, when produced by antibiotic treatment, still are sexually functional. In some Hawaiian populations of this species, males that are produced by antibiotic treatment appear to be completely sterile.

Having a genome of a sexual form of this species sequenced will allow us to determine the genes that are affected when a population becomes completely asexual, by later comparing the genome of asexual forms to that of the sexual form.

Data were generated by the Baylor College of Medicine's i5k pilot project.

View the Baylor College of Medicine's data sharing policy.

Community contact:

Image Credit: Trichogramma kaykai. Duotone Wageningen, The Netherlands. View Source. View Copyright
Organism Image
Feature Summary
The following features are currently present for this organism
Feature TypeCount
CDS71,714
mRNA13,200
Gene13,200
Contig N50
78771
Scaffold N50
3706225
Number Of Genes
13200
Community Contact
Community Contact: 
Richard Stouthamer|richards@ucr.edu
Image Credit
Trichogramma kaykai. Duotone Wageningen, The Netherlands. <a href="https://www.nsf.gov/news/mmg/media/images/sluipwesp_h.jpg">View Source.</a> <a href="https://www.nsf.gov/news/mmg/conditions.jsp?med_id=60117&from=">View Copyright</a>

Assembly Information

Analysis Name Whole genome assembly of Trichogramma pretiosum
Software Baylor College of Medicine genome assembly pipeline (NA)
Source Trichogramma pretiosum Tpre assembly
Date performed 2014-02-06
Materials & Methods

Sequence generation for assembly. For this project we are generating fairly high coverage in a number of different insert sized libraries. The assembly strategy is based around a seed allpaths assembly (the Broad Allpaths assembler) followed by seed assembly improvement using homegrown tools, Atlas-link and Atlas-GapFill, which can significantly improve the results. Thus we generate sequence data to enable the Allpaths assembly. As of Nov 2011 this is: - 40X genome coverage in 180bp insert library (100bp reads forward and reverse); and 40X 3kb insert data. To enable better scaffolding and local gap filling we additionally generate 500bp, 1kb, 2kb, and 8kb insert sizes at > 20X coverage.


Source: Baylor College of Medicine i5K Project Summary

Statistics

Assembly Metrics
Contig N50 78771
Scaffold N50 3706225
GC Content 38.10