Athalia rosae

Overview

No genome information of primitive hymenoptera (Symphyta, sawfly) is available vs higher suborder (Apocrita, Apis, Nasonia etc.). This species is a hymenopteran model maintained in laboratory through year.

Tools such that transgenesis, systemic RNAi, artificial egg activation, artificial fertilization by cryopreserved sperm injection, etc. are established.

DNA from a single haploid male (10-15 microgram/male) and from haploid offspring from a single mother (30-50 males) are available anytime.

Data were generated by the Baylor College of Medicine's i5k pilot project.

View the Baylor College of Medicine's data sharing policy.

Community contact:

Image Credit: Sanja565658. View Source. CC-BY-SA-3.0
Organism Image
Feature Summary
The following features are currently present for this organism
Feature TypeCount
CDS80,576
mRNA11,894
Gene11,894
Contig N50
51418
Scaffold N50
1366867
Number Of Genes
10784
Community Contact
Community Contact: 
Masatsugu Hatakeyama|sawfly@affrc.go.jp
Image Credit
Sanja565658. <a href="https://commons.wikimedia.org/wiki/File:Athalia_rosae_01.JPG">View Source.</a> <a href="https://creativecommons.org/licenses/by-sa/3.0/deed.en">CC-BY-SA-3.0</a>

Assembly Information

Analysis Name Whole genome assembly of Athalia rosae
Software Baylor College of Medicine genome assembly pipeline (NA)
Source Athalia rosae Aros01112013 assembly
Date performed 2013-07-16
Materials & Methods

Sequence generation for assembly. For this project we are generating fairly high coverage in a number of different insert sized libraries. The assembly strategy is based around a seed allpaths assembly (the Broad Allpaths assembler) followed by seed assembly improvement using homegrown tools, Atlas-link and Atlas-GapFill, which can significantly improve the results. Thus we generate sequence data to enable the Allpaths assembly. As of Nov 2011 this is: - 40X genome coverage in 180bp insert library (100bp reads forward and reverse); and 40X 3kb insert data. To enable better scaffolding and local gap filling we additionally generate 500bp, 1kb, 2kb, and 8kb insert sizes at > 20X coverage.


Source: Baylor College of Medicine i5K Project Summary

Statistics

Assembly Metrics
Contig N50 51418
Scaffold N50 1366867
GC Content 42.67
Manual Annotations