Mamestra configurata

Overview

Mamestra configurata (Walker) (Lepidoptera: Noctuidae) is commonly known as the bertha armyworm and is found primarily in western North America and Mexico. Adult moths are grey-black with a silvery-whitish reniform spot and fringe on each forewing. M. configurata overwinters as pupae in the soil with adults emerging from June until early August. The larvae are highly polyphagous and feed on a wide variety of broadleaved plants and some grasses . It is a pest of several important crops, including flax (Linum usitatissimum), sweet clover (Melilotus spp.), alfalfa (Medicago sativa) and canola (Brassica napus and B. rapa). It is a periodic, but serious pest of canola with outbreaks occurring every 7-10 years and spanning the entire Canadian prairies. The larvae initially feed on canola leaves, but later may feed on pods before dropping to the ground in late August to pupate. Control of the pest in crops is generally achieved through the use of insecticides, although naturally-occurring biological agents (bacteria, viruses, parasitoids) are believed to be responsible for curtailing outbreak populations. The status and distribution of M. configurata and other pests is monitored annually be the Prairie Pest Monitoring Network. Research on M. configurata has focused on host plant selection, population genetics, genomics, biological control agents, pheromone biology, and digestive physiology/biochemistry. The M. configurata genome is approximately 590 Mb and is the largest noctuid genome reported to date.

Community contact:

Image Credit: Dr. Tyler Wist CC-BY.
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Mamestra configurata (Walker) (Lepidoptera: Noctuidae) is commonly known as the bertha armyworm and is found primarily in western North America and Mexico. Adult moths are grey-black with a silvery-whitish reniform spot and fringe on each forewing. M. configurata overwinters as pupae in the soil with adults emerging from June until early August. The larvae are highly polyphagous and feed on a wide variety of broadleaved plants and some grasses . It is a pest of several important crops, including flax (Linum usitatissimum), sweet clover (Melilotus spp.), alfalfa (Medicago sativa) and canola (Brassica napus and B. rapa). It is a periodic, but serious pest of canola with outbreaks occurring every 7-10 years and spanning the entire Canadian prairies. The larvae initially feed on canola leaves, but later may feed on pods before dropping to the ground in late August to pupate. Control of the pest in crops is generally achieved through the use of insecticides, although naturally-occurring biological agents (bacteria, viruses, parasitoids) are believed to be responsible for curtailing outbreak populations. The status and distribution of M. configurata and other pests is monitored annually be the Prairie Pest Monitoring Network. Research on M. configurata has focused on host plant selection, population genetics, genomics, biological control agents, pheromone biology, and digestive physiology/biochemistry. The M. configurata genome is approximately 590 Mb and is the largest noctuid genome reported to date.

Scaffold N50
207752
Community Contact
Community Contact: 
Cathy Coutu|Cathy.Coutu@AGR.GC.CA
Image Credit
Dr. Tyler Wist <a rel="license" href="http://creativecommons.org/licenses/by/4.0/">CC-BY</a>.

Assembly Information

Analysis Name Mamestra configurata genome assembly ASM219265v1 (GCA_002192655.1)
Software SOAPdenovo2 (SOAPdenovo2 version 2.3)
Source SAMN06637963
Date performed 2018-06-22
Materials & Methods DNA extracted from 3 male siblings from a long-established (> 35 years in culture), highly inbred laboratory colony was used to generate 6 Illumina paired-end gDNA libraries (insert sizes ranging from 300bp to 10kb) and 4 Roche 454 paired-end gDNA libraries (insert sizes ranging from 15-40 kb). These libraries were sequenced at the National Research Council, Plant Biotechnology Institute (Saskatoon, Saskatchewan, Canada) by Illumina HiSeq or Roche 454 FLX-titanium pyrosequencing, respectively. All reads were trimmed for adapters and quality, and duplicate reads and orphaned reads were removed using Trimmomatic-0.30. Mitochondrial DNA reads and PhiX reads were identified and removed by mapping all reads to the Spodoptera litura mitochondrial genome (NC_022676.1) and the PhiX genome (NC_001422.1), respectively, using the CLC genomics workbench, and error correction using SOAPec was applied to all remaining reads. Assembly was done using SOAPdenovo2, with a kmer of 63 for contig assembly and 47 for scaffold assembly. Gaps between contigs were filled or partially filled by running two iterations of GapCloser on the assembled scaffolds. Small scaffolds which were >95% identical to portions of larger scaffolds were removed using the CLC genomics workbench 9.0.1. Scaffolds less than 500 bp long were removed.

Statistics

Assembly Metrics
Contig N50
Scaffold N50 207752
GC Content 37.15